Descrição
For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation.
The FlexiGene DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Easy handling, no risk of sample mix-up
- Scalable purification from 0.1–20 ml whole blood
- Direct purification from whole blood, buffy coat, and cultured cells
- Fast procedure
- No organic extraction
Product Details
The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.
Principle
The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.
Applications
DNA purified using the FlexiGene DNA kit performs well downstream applications, including,
- PCR
- Southern blotting
- Archiving
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR techniques, restriction digest, Southern blotting, sequencing |
| Sample amount | 100 µl – 20 ml |
| Format | Scalable |
| Time per run or per prep | 25 – 45 minutes (+ DNA rehydration) |
| Main sample type | Whole blood, buffy coat, cultured cells |
| Technology | Single tube precipitation method |
| Processing | Manual |
| Yield | 35 µg / ml whole blood |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Elution volume | 200 µl – 1000 µl |
