Descrição
Target Name
D-Dimer,D2D
Abbreviation
D2D
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Typical Data
| These standard curves are provided for demonstration only.
A standard curve should be generated for each set of samples assayed. |
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Materials provided
- A micro ELISA plate — The 96-well plate has been pre-coated with an anti-human D2D antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard — Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled D2D antibody (100 x concentrate) (120 μl/bottle) —Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) — Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) —Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) —Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)—Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) — Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) — Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) — Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) — Cover the microplate when incubation.
- An instruction manual
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
Storage
Store at 2-8°C. Please refer to protocol.
Description
This Human D-Dimer (D2D) ELISA Kit is suitable for qualitatively determining D2D concentrations in multiple biological fluids, including human serum and plasma in vitro. D-dimer is the final degradation product of cross-linked fibrin generated during plasma coagulation. Yumeng Yao etc. demonstrated that D-dimer expression is commonly elevated in patients infected with SARS-CoV-2. D-dimer levels correlate with disease severity and are a reliable prognostic marker for in-hospital mortality in patients admitted for COVID-19.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human D2D in the sample. Standards and samples are respectively added to the microplate wells pre-coated with anti-human D2D antibody. Biotin-labeled D2D antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. D2D in the sample or standard is captured by the immobilized antibody and then binds to the biotinylated D2D monoclonal antibody. And the biotin on the biotinylated D2D antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of TMB substrate. The addition of the stop solution into the wells immediately turns the blue solution into a yellow one. The concentration of D2D in the samples is directly proportional to the OD value (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See the manual for more information on validation.
